- Introduction to HPLC
- HLPC:
- System components
- Method of analysis
- Method development
- Method verification
- HPLC validation:
Validation protocol
Validation parameters
Acceptance criteria
Deviation reporting
Final report
- Workshop (Developing HPLC testing method validation protocol)
- Common problems and the recommended actions
A- Physical problems:
1- Abnormal Pressure
No Pressure Reading, No Flow
No Pressure Reading, Flow Is Normal
Steady High Pressure
Steady Low Pressure
Pressure Climbing
Pressure Dropping to Zero
Pressure Dropping, But Not to Zero
Pressure Cycling
2- Leaks
Leaky Fittings
Leaks at Pump
Injector Leaks
Column Leaks
Detector Leaks
3- Problems with the injector
Manual Injector, Hard to Turn
Manual Injector, Hard to Load
Autoinjector,Will Not Turn
Autoinjector, Other Problems
Workshop (Discussing real problems and solutions)
B- Problems with the chromatogram
Peak Tailing
Peak Fronting
Split Peaks
Distortion of Larger Peaks
Distortion of Early Peaks
Tailing, Early Peaks More Than Later Ones
Increased Tailing
Acidic or Basic Peaks Tail
Extra Peaks
Retention Time Drifts
Abrupt Retention Time Changes
Baseline Drift
Baseline Noise (Regular)
Baseline Noise (Irregular)
Broad peaks
Loss of Resolution
All Peaks Too Small
All Peaks Too large
Other technical HPLC problems, source and recommended solutions
Workshop (Discussing real problems and solutions)
Course evaluation
18(hours)